TABLE 4.
Primers and probes for RT-PCR
| Gene | Oligonucleotide | Primer/probe | Sequence 5′ to 3′ |
|---|---|---|---|
| Subgenomic Envelope (E) | sgLeadSARSCoV2-F | Forward Primer | CGATCTCTTGTAGATCTGTTCTC |
| E_Sarbeco_R | Reverse Primer | ATATTGCAGCAGTACGCACACA | |
| E_Sarbeco_P1 | Probe | VIC-ACACTAGCCATCCTTACTGCGCTTCG-MGBNFQ | |
| Envelope (E) | E_Sarbeco_F | Forward Primer | ACAGGTACGTTAATAGTTAATAGCGT |
| E_Sarbeco_R | Reverse Primer | ATATTGCAGCAGTACGCACACA | |
| E_Sarbeco_P1 | Probe | FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ1 | |
| Nucleocapsid (N) | 2019-nCoV_N1-F | Forward Primer | GACCCCAAAATCAGCGAAAT |
| 2019-nCoV_N1-R | Reverse Primer | TCTGGTTACTGCCAGTTGAATCTG | |
| 2019-nCoV_N1-P | Probe | FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1 | |
| ORF1ab | SARS-CoV2.ORF1ab.F | Forward Primer | GGCCAATTCTGCTGTCAAATTA |
| SARS-CoV2.ORF1ab.R | Reverse Primer | CAGTGCAAGCAGTTTGTGTAG | |
| SARS-CoV2.ORF1ab.P | Probe | FAM-ACAGATGTCTTGTGCTGCCGGTA-BHQ1 |