Fig. 7.
Dose-dependent intracellular cAMP production in rat primary esophageal cells and CRF2b-transfected HEK-293 cells stimulated with CRF and Ucn 2. Rat primary esophageal mucosal cells or CRF2b-transfected HEK-293 cells (1–2×105 cells/well) grown on 12-well plates were treated with increasing concentrations of CRF or Ucn 2 (10−10 to 10−6m) in the presence of 1 mm 3-isobutyl-1-methyl-xanthine for 30 min. CRF2-selective antagonist, astressin2-B, at a concentration of 1 μm was added concurrently to a separate set of Ucn-2 treated cells. Results are expressed as mean ± se of increases over basal unstimulated levels (1.0 ± 0.2 pmol and 2.4 ± 0.3 pmol per 15 min per 105 cells for the esophageal cells and HEK-293 cells, respectively).