Fig. 2.

Distribution of the LM1 extensin epitope in Arabidopsis thaliana p33*p34 double mutant and WT root tips. Immunolabelling was performed on 9-d-old roots using the anti-extensin monoclonal antibody LM1. Roots were either elicited with 1 μm Flg22 or not elicited. Images are 3-D reconstructions of 1-μm section stacks and were obtained with a Leica SP2 inverted confocal laser scanning microscope (λ _excitation, 488 nm; λ _emission, 507–550 nm). For each condition, five plant root tips were observed. Immunolabelling was performed as described in Castilleux et al. (2020). Scale bars = 50 μm. RT, root tip; BLC + M, border-like cells and mucilage. Flg22: flagellin 22.