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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: J Immunol. 2020 Aug 5;205(5):1217–1227. doi: 10.4049/jimmunol.1901167

Figure 2. Effector cytokine response during infection is regulated by TcR signal strength independent of antigen affinity.

Figure 2.

Cells from blood samples collected each week were cultured with WT OVA peptide (N4) or PMA/Ionomycin in the presence of BFA and cells analyzed for cytokine production by flow cytometry for TNFα (A) and IFNγ (B). As a positive control (C), TNFα (top panel) and IFNγ (bottom panel) percent and MFI were quantified after stimulation with PMA/Ionomycin at day 7. Double positive producers of TNFα+ and IFNγ+ were quantified (D) and PMA/Ionomycin control (E) on day 7. *p>0.05 based upon one-way and two-way ANOVA. Data (mean ± SEM) is representative of two independent experiments with n ≥ 3–4. n=4 in WT and Itk−/− group receiving LM-N4; n=3 for WT group receiving LM-T4; n=4 for Itk−/− group receiving LM-T4.