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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: J Immunol. 2020 Aug 5;205(5):1217–1227. doi: 10.4049/jimmunol.1901167

Figure 6. Co-transfer of WT and Itk−/− cells eliminates the increase in memory precursor effector cells.

Figure 6.

Adoptive transfer of mixed WT (CD45.2+CD45.1+) and Itk−/− (CD45.2+CD45.1−) OT-1 cells into single CD45.1 recipient mice was carried out similarly to figure 1A and followed by infection with LM-N4 and number of transferred donor cells determined (A). (B,C) The percentages of CD127loKLRG1hi (SLECs) and CD127hiKLRG1lo (MPECs) was determined. (D) Production of either TNFα or IFNγ (E) were quantified along with the corresponding MFI (F). Percentage of dual production of TNFα/IFNγ (G). *p<0.05 and n.s. = “Not Significant” based upon two-way ANOVA. Data (mean ± SEM) is representative of two independent experiments with n=5 for each group.