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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: J Immunol. 2020 Aug 5;205(5):1217–1227. doi: 10.4049/jimmunol.1901167

Figure 7. Inflammation negatively regulates memory precursor effector cell development.

Figure 7.

Adoptive transfer of OT-1 WT or Itk−/− cells into single CD45.1 recipient mice was carried out similarly to figure 1A and followed by infection with LM-N4 and injection of CpG to induce inflammation. (A) The number of transferred OT-1 cells in blood was quantified. The percent of SLECs (B) and MPECs (C) was assessed. Percentage of cells producing TNFα (D) and IFNγ pro-inflammatory cytokine and MFI (E,G). Cells producing both TNFα and IFNγ (F). *p<0.05., n.s. = “Not Significant” based on two-way ANOVA. Data (mean ± SEM) is representative of two independent experiments n=5 for WT and Itk−/− group receiving LM-high affinity OVA (N4), n=3 for WT and Itk−/− group receiving LM-high affinity OVA (N4) and CpG.