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Chemical structure and cytotoxicity of gamabufotalin (CS-6) on osteoclast precursor cells (bone marrow–derived monocyte–macrophage (BMM) lineage). (A, B) Chemical structure of CS-6. (C–E) Cell viability of BMMs as evaluated by CCK-8 assay following treatment with or without indicated concentrations of CS-6 at the time point of (C) 24, (D) 48, and (E) 96 h, respectively. Values were presented as the mean ± standard deviation (n = 5); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
