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. 2021 Apr 12;95(9):e01939-20. doi: 10.1128/JVI.01939-20

TABLE 1.

m6A peaks in VSV RNAs

VSV RNAa Peak no. Peak range (nt)b Gene locationc Peak size (nt) Fold enrichmentd Putative m6A site(s)e
Genome 1 898–1047 N 149 2.57 AAACC
2 1944–2143 P 199 5.26 GAACA, AGACA
3 2891–3040 M 149 20.7 GAACA, GAACA
4 3389–3588 G 199 6.16 GAACA, GAACA, AAACA, GAACT
5 3838–4036 G 198 22.56 AGACC, AAACC
6 7426–7475 L 49 2.10 NA
Antigenome 1 1–449 N 448 11.99 AAACT, AGACA, AAACT, GAACT, AGACA
2 499–548 N 49 3.51 NA
3 599–698 N 99 2.39 GAACA, GAACA
4 898–997 N 99 2.84 AGACT, AGACA
5 1147–1246 N 99 2.55 AAACC
6 1396–1894 P 498 43.8 AAACC, GAACA, GGACT, GGACT, GAACT
7 1944–1993 P 49 6.79 AGACC, AAACA
8 2742–2890 M 148 4.50 AAACA, AGACC
9 3389–3488 G 99 3.64 NA
10 3688–4036 G 348 10.26 AGACC, AGACT, GAACC, AGACA, GAACC
11 4685–4784 L 99 3.62 GAACT, GGACT
12 4884–4933 L 49 2.57 NA
13 5582–5681 L 99 3.13 GGACT
14 6678–6727 L 49 2.46 GAACA
15 7575–7624 L 49 7.38 GGACT
16 8323–8471 L 148 11.02 GAACC, AAACC, AGACC, GAACC
17 8771–8820 L 49 2.02 NA
18 8970–9119 L 149 2.08 AAACC, AGACT
19 10615–10664 L 49 4.80 AAACT
20 10764–10863 L 99 10.25 GGACC
21 10914–11062 L 148 2.71 AGACC
mRNAs 1 51–249 N 198 5.18 AGACA, AAACT, GAACT, AGACA
2 1346–1445 gs, ge, and ig 99 5.73 GGACT, GAACT
3 1496–1595 P 99 2.01 GAACA, GGACT
4 1645–1844 P 199 6.62 AAACC
5 4685–4784 G, gs, ge, ig, L 99 12.64 GAACT, GGACT
6 8372–8471 L 99 2.06 GAACC, AAACC, AGACC, GAACC
a

Genome and antigenome were extracted from highly purified virions grown in A549 cells. VSV mRNAs were extracted from VSV-infected A549 cells and purified by poly(A) bead.

b

Nucleotide sequence position with reference to the VSV Indiana strain (GenBank accession no. J02428). Nucleotide ranges are indicated.

c

The VSV genes and regulatory elements are covered by m6A peaks. These regions may contain m6A sites. VSV gene start, gene end, and intergenic sequence are indicated by gs, ge, and ig, respectively.

d

Log2 enrichment of the m6A peaks identified in the VSV genome, antigenome, and mRNAs.

e

Putative m6A sites are identified based on searching for the presence of the m6A motif Pu [G > A]m6AC[A/C/U] (Pu represents purine) in the peak ranges. NA indicates no known m6A motifs are found in this region.