TABLE 2.

m⁶A peaks in Sendai virus RNAs

SeV RNA peak no.a	Peak range (nt)b	Gene locationc	Peak size (nt)	Fold enrichmentd	Putative m6A site(s)e
1	351–450	N	99	1.34	AGACC
2	1401–1750	N	349	2.63	AAACA, GGACC, AAACC, AGACA, AGACT, AAACT
3	2101–2500	P	399	2.37	GAACA, AAACC, AAACA, GAACT, AAACT, AGACT, AAACA, AGACC, GGACT, GAACT
4	2651–2750	P	99	1.56	GGACA, AGACC, AAACC, GGACC
5	3251–3600	P	349	3.35	AAACC, GGACC, GGACA, GAACC, AGACA, AGACC, GAACT, GGACA
6	5301–5400	GFP	99	1.39	GAACT

^aTotal RNA was extracted from rSeV-GFP or mock-infected A549 cells and subjected to sonication. RNA containing m⁶A methylation was pulled down by m⁶A antibody. Three replicates (n = 3) of RNA from virus-infected cells were m⁶A sequenced.

^bNucleotide sequence position with reference to the Sendai virus Z strain expressing GFP (GenBank accession no. AB855655). Nucleotide ranges are indicated.

^cThe SeV genes and GFP gene are covered by m⁶A peaks. These regions may contain m⁶A sites.

^dLog₂ enrichment of the m⁶A peaks identified in the SeV antigenome and mRNAs.

^ePutative m⁶A sites are identified based on searching for the presence of the m⁶A motif Pu [G > A]m⁶AC[A/C/U] (where Pu represents purine) in the peak ranges.