FIG 8.

Surface expression of IFNAR1/2 on HSA⁺ and HSA⁻ MDM. (A) Flow cytometry gating strategy and representative histograms (filled gray, PE FMO control; filled light blue, HSA⁻ MDM; empty red, HSA⁺ MDM) depicting IFNAR1 and IFNAR2 expression on HSA⁻ and HSA⁺ MDM. Intact cells were gated (black), after which HSA⁻ (blue) and HSA⁺ (red) MDM were defined. Histogram peak counts (y axis) for HSA⁻ and HSA⁺ populations were normalized to that of the PE FMO control for visualization purposes. (B) IFNAR1 expression on HSA⁻ (blue) and HSA⁺ (red) MDM, as measured by mean fluorescent intensity (n = 7). (C) IFNAR2 expression on HSA⁻ and HSA⁺ MDM, as measured by mean fluorescent intensity (n = 7). Data represent mean ± SEM; n values represent separate biological replicates.