Skip to main content
. 2021 Apr 23;12:644159. doi: 10.3389/fendo.2021.644159

Figure 2.

Figure 2

High insulin and glucose concentrations regulate the activity of PTEN/AKT/eNOS MAPK/ET-1 pathways. (A): Westernblot analysis of PTEN, AKT, P-AKT, eNOS, P38 MAPK, P-P38 MAPK and ET-1 expression in HGECs treatment with different concentrations of glucose and 0ng/ml concentration of insulin for 48h. (B, C): Quantification of result in (A). (D): Westernblot analysis of PTEN, AKT, P-AKT, eNOS, P38, P-P38 and ET-1 expression in HGECs treatment with different concentrations of insulin and 5.5mmol/L concentration of glucose for 48h. (E, F): Quantification of result in (D). Data are reported as mean ± SD *P < 0.05. All data are representative of three independent experiments. OC, osmotic control group; Glu, glucose; Ins, insulin; PTEN, phosphatase and tensin homologue on chromosometen; P-AKT, phospho-AKT; P38-MAPK, p38 mitogen-activated protein kinase; P-P38 MAPK, phospho-p38 mitogen-activated protein kinase; eNOS, endothelial nitric oxide synthase; P-eNOS, phospho-endothelial nitric oxide synthase; ET-1, endothelin-1.