Fig. 4.

Mapping glycation on hACE2. a Human ACE2 sequence (www.uniprot.org; entry: Q9BYF1, entry name: ACE2_HUMAN) showing in red the glycated lysine residues obtained after 12 days of incubation with 120 mM of glucose. b Position glycated lysine (K) after 12 days of incubation with 12 mM, 60 mM, and 120 mM of glucose and function of glycated sites. c Human ACE2 homodimer (PDB 1r42) showing the lysine 353 (K353), involved in the Spike-RBD binding to ACE2, lysine 470 (K470) (unknown function). ACE2 structure from PDB 6M17 showing the glycated lysine 619 (K619), 631 (K631), 659 (K659), and 689 (K689) in the polar neck region involved in the dimerization of ACE2