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. 2021 May 7;7(19):eabe8349. doi: 10.1126/sciadv.abe8349

Fig. 7. EXT1 k.d. results in increased secretory cargo trafficking.

Fig. 7

(A) SAFE analysis of EXT1 interactome in ER microsomes. (B) Schematic representation of the SILAC workflow. (C and D) Up-regulated (C) and down-regulated (D) proteins involved in anterograde and retrograde transport. (C) CAMSAP2, calmodulin regulated spectrin associated protein family member 2; CLIP1, CAP-gly domain-containing linker protein 1; KIF23, kinesin family member 23; SCFD2, Sec1 family domain containing 2. (D) DNAH2, dynein axonemal heavy chain 2; DNMBP, dynamin binding protein; DYNC1LI2, dynein cytoplasmic 1 light intermediate chain 2; DYNLT3, dynein light chain tctex-type 3; GORASP1, Golgi reassembly stacking protein 1; MAP1A, microtubule associated protein 1A; MAP7D1, MAP7 domain containing 1; MYO1C, myosin IC; STX8, syntaxin 8. One-way ANOVA, *P < 0.05, ***P < 0.001, ****P < 0.0001. (E) Live imaging of retention selective hook (RUSH)–synchronized traffic of tumor necrosis factor (TNF) protein in HeLa cells. Scale bars, 10 μm. (F) Mean normalized fluorescence intensity (a.u.) after the addition of biotin. Two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli. See also fig. S7.