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. 2021 May 7;7(19):eabf7114. doi: 10.1126/sciadv.abf7114

Fig. 3. Sensitivity to PRDX4 depletion is associated with increased ROS abundance.

Fig. 3

(A) Flow cytometry histograms of cellular ROS measured by oxidized CellROX in cells sensitive (MIA PaCa-2 and PANC-1) and resistant (Capan-2 and GP-3A) to PRDX4 depletion 72 hours after transfection with siRNA targeting nothing (NTC) or PRDX4 (PRDX4 kd). (B) Quantification of relative fluorescence intensity from samples as shown in (A). Menadione (100 μM) exposure for 1 hour was used as a positive control. Each data point represents an independent experiment; bars represent the average value ± SD (**P < 0.01; *P < 0.05).