Fig. 2. Fungi-derived α-mannans induced Dectin-2/3–mediated phosphorylation of c-Cbl to control susceptibility of mice to colitis.

(A and B) Immunoblotting analysis of α-mannan–induced phosphorylation of c-Cbl and Syk in BMDCs isolated from wild-type (Clec4n^+/+), Decin-2–deficient [Clec4n^−/−, (A)], or Decin-3–deficient [Clec4d^−/−, (B)] mice. (C and D) Immunoblotting analysis of α-mannan–induced phosphorylation of c-Cbl and Syk in wild-type BMDCs (C) or human PBMCs (D), which were pretreated with or without Syk inhibitor R406 for 30 min before stimulation. (E to H) Fungal burden (E), weight loss (F), colon length (G), and histological analysis (H) of DSS (2.5%)–treated Cbl^f/fCD11c^Cre/+ and c-Cbl^f/f mice (n = 4 to 7 mice group in each experiment), which were pretreated with or without fluconazole (0.5 mg/ml) in drinking water. Bar graphs show means ± SEM. *P < 0.05 and **P < 0.01. Photo credit: Jie-Lin Duan, Tongji University.