Fig. 5. Resting integrin α₅β₁ is disadvantageous for FN binding.

(A) An overlay of the βI, hybrid, and PSI domains of resting (blue) and FN-bound integrin (gray) shows the movement of the α₁ and α₇ helices in the βI domain and the subsequent swing-out motion of the hybrid domain. (B) ADMIDAS is farther from FN10 (Y1446) in the resting βI domain (blue) than in the open βI domain (gray). (C) Comparison of the position of glycan^N343 on β₁ with (top) and without (bottom) ligand binding. The glycan^N343 hinders the FN binding in the resting structure.