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. 2021 Jan 20;13(2):128–140. doi: 10.1093/jmcb/mjaa079

Figure 2.

Figure 2

NRF1 and p65 are synchronously upregulated in CSE-, LPS-, PAM3CSK4-, or their combinations-treated L132 cells. (A) L132 cells were treated with CSE at indicated concentrations for 48 h. Cell viability was detected through MTT assay. (B and C) L132 cells were treated with 2% CSE for 42 h, followed by 1 μg/ml LPS or 10 ng/ml PAM3CSK4 for 6 h. Cells were stained with p65 antibody to observe the nuclear translocation and the intensity was quantified. (D‒G) Protein levels of NRF1, p65, and IL-6 were determined by western blotting. (H‒K) mRNA expression levels of NRF1, RELA, IL6, and TNFA were determined by qRT-PCR. Mean ± SD, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 by one-way ANOVA.