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. 2021 May 7;10:e64711. doi: 10.7554/eLife.64711

Figure 3. RNAseq analysis of A549 cells cultured in the presence of sera from bonded (B), bond-disrupted (BD), or virgin (V) P. californicus.

(a) Bar graphs showing number of differentially expressed genes in each pairwise comparison group. (b ) Volcano plots showing differentially expressed genes between the B vs BD, and B vs V groups. (c) Venn Diagrams showing overlapping differentially expressed genes. The identity of genes is shown in the right. In the bonded group, mice were paired for 12 months. For the bond-disrupted group, we separated paired mice after 12 months of bonding, and collected the sera 1 week after bond disruption. For virgin mice, we collected sera from mice housed 3/cage.

Figure 3.

Figure 3—figure supplement 1. CSC markers in A549 cells cultured in vitro and tumors.

Figure 3—figure supplement 1.

Figure 3—figure supplement 2. Unsupervised hierarchical clustering based on log2 transformed values on all RNAseq data.

Figure 3—figure supplement 2.

Figure 3—figure supplement 3. RNAseq analysis summary of A549 cells.

Figure 3—figure supplement 3.