Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (species) (Schmidtea mediterranea) | Asexual clonal line CIW4 | Inhouse laboratory cultured | All animals used in this study | |
| Gene (Schmidtea mediterranea) | RAD51 | GenBank | KM487300.1 | RNAi |
| Gene (Schmidtea mediterranea) | BRCA2 | GenBank | KT375435.1 | RNAi |
| Gene (Schmidtea mediterranea) | ATR | Planmine | dd_Smed_v6_8754_0_1 | RNAi |
| Gene (Schmidtea mediterranea) | ATM | Planmine | dd_Smed_v6_14586_0_1 | RNAi |
| Gene (Schmidtea mediterranea) | FANC-J | Planmine | dd_Smed_v6_16638_0_2 | RNAi |
| Gene (Schmidtea mediterranea) | PARP1 | Planmine | dd_Smed_v6_10338_0_1 | RNAi |
| Gene (Schmidtea mediterranea) | PARP2 | Planmine | dd_Smed_v6_6154_0_1 | RNAi |
| Gene (Schmidtea mediterranea) | PARP3 | Planmine | dd_Smed_v6_2611_0_1 | RNAi |
| Antibody | Anti-digoxigenin-POD, Fab fragments (rabbit polyclonal) | Sigma/Roche | # 11207733910 RRID:AB_514500 |
FISH (1:2000) |
| Antibody | Anti-fluorescein-POD, Fab fragments (rabbit polyclonal) | Sigma/Roche | #11426346910 RRID:AB_840257 |
FISH (1:2000) |
| Antibody | Anti-H3P (phosphorylated serine 10 on histone H3 (rabbit polyclonal)) |
Millipore | #09–797 RRID:AB_1977177 |
IF (1:1000) |
| Antibody | Anti-Poly (ADP) Ribose (PAR) monoclonal antibody (clone 10H) (mouse monoclonal) | Santacruz | #SC-56198 RRID:AB_785249 |
IF (1:250) |
| Antibody | Anti-TUDOR-1(Tud1) (rabbit polyclonal) | Aboobaker Lab Solana et al., 2009 | IF (1:250) | |
| Antibody | Goat-Anti-rabbit-HRP (goat polyclonal) | Invitrogen | #65–6120 | IF (1:2000) |
| Antibody | Goat-Anti-mouse HRP (goat polyclonal) |
Invitrogen | #62–6520 | IF (1:2000) |
| Chemical compound, drug | Formaldehyde | EMD Millipore | FX0410-5 | Used at 4% for fixing animals |
| Chemical compound, drug | Platinum Taq | Invitrogen | #10966026 | PCR |
| Chemical compound, drug | Trizol | Invitrogen | #15596026 | RNA isolation |
| Chemical compound, drug | Superscript III Reverse transcriptase | Invitrogen | #18080093 | cDNA synthesis |
| Chemical compound, drug | Absolute qPCR mix, SYBR Green | Thermo Fisher | #AB1159A | RT-qPCR expression |
| Chemical compound, drug | Chloretone | Sigma | #112054 | Anaesthetising worms |
| Chemical compound, drug | Instant ocean Sea Salt | Instant Ocean | #SS15-10 | Culturing animals |
| Sequence-based reagent | ATR_F | Sigma | GCGCAGGAATTCAGAAACTC | dsRNA for RNAi |
| Sequence-based reagent | ATR_R | Sigma | GACGGTCACCGAGACCTAAA | dsRNA for RNAi |
| Sequence-based reagent | ATM_F | Sigma | ATTCACTGGGCCAACGTTGA | dsRNA for RNAi |
| Sequence-based reagent | ATM_R | Sigma | TCTTCCCTCGACACCAAACG | dsRNA for RNAi |
| Sequence-based reagent | BRCA2_F | Sigma | ATGGACGGGATGTGATGAGC | dsRNA for RNAi |
| Sequence-based reagent | BRCA2_R | Sigma | ATGCACCTTCCACGAGCAAT | dsRNA for RNAi |
| Sequence-based reagent | Rad51_F | Sigma (Peiris et al., 2016) | TTTGCAAGGTGGTGTTGAAA | dsRNA for RNAi |
| Sequence-based reagent | Rad51_R | Sigma (Peiris et al., 2016) | ATCAGCCAACCGTAACAAGG | dsRNA for RNAi |
| Sequence-based reagent | FancJ_F | Sigma | AGCGGAAAGGAAGACTGTCA | dsRNA for RNAi |
| Sequence-based reagent | FancJ_R | Sigma | TAGGCACGACTTCACTGCAC | dsRNA for RNAi |
| Sequence-based reagent | PARP1_F | Sigma | AACGTGCAATGCTGGAGTTT | dsRNA for RNAi |
| Sequence-based reagent | PARP1_R | Sigma | TCCTACCCCTTTGCAACTGT | dsRNA for RNAi |
| Sequence-based reagent | PARP2_F | Sigma | TGACTGGCAAGATCGTCAGA | dsRNA for RNAi |
| Sequence-based reagent | PARP2_R | Sigma | AGTTGTTCTTGAACCGTGCC | dsRNA for RNAi |
| Sequence-based reagent | PARP3_F | Sigma | AACTCTTGTGGCATGGAACC | dsRNA for RNAi |
| Sequence-based reagent | PARP3_R | Sigma | CGCAGAGTTCGTGAAATGAA | dsRNA for RNAi |
| Sequence-based reagent | Atr_qPCR_F | Sigma | ACGCGTGGTATAGGAGCGTG | qPCR |
| Sequence-based reagent | Atr_qPCR_R | Sigma | TATGACGGTCACCGAGACC | qPCR |
| Sequence-based reagent | Atm_qPCR_F | Sigma (Peiris et al., 2016) | CTGATTGGTCGGCTTTCATT | qPCR |
| Sequence-based reagent | Atm_qPCR_R | Sigma (Peiris et al., 2016) | AGCTAACCAATCCCCCAAAG | qPCR |
| Sequence-based reagent | Brca2_qPCR_F | Sigma (Peiris et al., 2016) | CAAAGAGACCCTGCTTGAGG | qPCR |
| Sequence-based reagent | Brca2_qPCR_R | Sigma (Peiris et al., 2016) | AGCCGGAACACAGTACCATC | qPCR |
| Sequence-based reagent | Rad51_qPCR_F | Sigma (Peiris et al., 2016) | ATGTCAGAATCCCGATACGC | qPCR |
| Sequence-based reagent | Rad51_qPCR_R | Sigma (Peiris et al., 2016) | ATCAGCCAACCGTAACAAGG | qPCR |
| Sequence-based reagent | FancJ_qPCR_F | Sigma | CACCAGTGGAACCTTATCTCC | qPCR |
| Sequence-based reagent | FancJ_qPCR_R | Sigma | GGACGGTCCGTTTCCGATGCT | qPCR |
| Sequence-based reagent | Parp1_qPCR_F | Sigma | CGATTCTATACAATGATGCC | qPCR |
| Sequence-based reagent | Parp1_qPCR_R | Sigma | CTGCTTCCATCAGTTTATAGGC | qPCR |
| Sequence-based reagent | Parp2_qPCR_F | Sigma | CAAGAACAACTAATTACGGTGG | qPCR |
| Sequence-based reagent | Parp2_qPCR_R | Sigma | GATCTCGTCGGGTAATATAG | qPCR |
| Sequence-based reagent | Parp3_qPCR_F | Sigma | GATATTGAAAGTACTCAAGC | qPCR |
| Sequence-based reagent | Parp3_qPCR_R | Sigma | CAACATCTAGCATCTTGAACC | qPCR |
| Software algorithm | TBLASTX | U.S. National Library of Medicine | RRID:SCR_011823 | Human gene comparison |
| Software algorithm | BLASTX | U.S. National Library of Medicine | RRID:SCR_001653 | Homology searches |
| Software algorithm | eggNOG.5.0 | European Molecular Biology Laboratory, Hiedelberg | RRID:SCR_002456 | Identify orthologs |
| Software algorithm | Inparanoid | http://inparanoid.sbc.su.se/cgi-bin/index.cgi | RRID:SCR_006801 | Identify orthologs |
| Software algorithm | Planmine | MPI-CBG, Dresden (Dr. Jochen Rink) | http://planmine.mpi-cbg.de/ | Identify flatworm sequences |
| Software algorithm | Fiji/Image-J | MPI-CBG, Dresden/National Institutes of Health (NIH) | PMID:22743772
RRID:SCR_002285 |
Image processing and analysis |
| Software algorithm | KOMET (andor) | Oxford instruments | https://andor.oxinst.com/products/komet-software/ | Comet assay analysis |
| Software algorithm | Graphpad Prism v6 | Graphpad | RRID:SCR_002798 | Graphs and statistical analysis |
| Software algorithm | Illustrator CC | Adobe | RRID:SCR_010279 | Making figures |