| Antibody |
Mouse monoclonal anti-TTF-1 (clone 8G7G3/1) |
Agilent Dako |
IR05661-2 |
(1:200) |
| Antibody |
Rabbit polyclonal anti-SARS-CoV nucleocapsid (N) protein |
Rockland |
200–401-A50 |
(1:2000) |
| Antibody |
Goat polyclonal anti-rabbit IgG- Alexafluor 568 |
Thermo Fisher |
A11011 |
(1:1000) |
| Biological sample (virus) |
2019-nCoV (SARS-CoV-2)/USA-WA1/2020 |
Center for Disease Control, BEI |
MN985325 |
|
| Biological sample (Homo sapiens) |
Formalin-fixed, paraffin-embedded, autopsy lung tissue (39 deceased patients or ‘decedents’) |
Brigham and Women’s Hospital, Autopsy Division |
N/A |
|
| Chemical compound, drug |
Dulbecco’s Modified Eagle Medium (DMEM), High Glucose, Pyruvate |
Thermo Fisher Scientific |
11995065 |
|
| Chemical compound, drug |
Eagle’s Minimum Essential Media (EMEM) |
ATCC |
ATCC 30–2003 |
|
| Chemical compound, drug |
Opti-MEM Reduced Serum Medium |
Thermo Fisher |
31985062 |
|
| Chemical compound, drug |
Penicillin-Streptomycin (10,000 U/mL) |
Thermo Fisher |
15140122 |
|
| Chemical compound, drug |
Fetal Bovine Serum, Inactivated |
Atlanta Biologicals |
S11150H |
|
| Commercial assay or kit |
In-Fusion HD Cloning Plus |
Takara Bio |
638910 |
|
| Chemical compound, drug |
Lipofectamine 3000 Transfection Reagent |
Thermo Fisher Scientific |
L3000008 |
|
| Chemical compound, drug |
Polybrene |
Sigma-Aldrich |
TR-1003-G |
|
| Chemical compound, drug |
Paraformaldehyde (16%) |
Electron Microscopy Services |
15710 |
|
| Chemical compound, drug |
Phusion High-Fidelity DNA Polymerase |
New England Biolabs |
M0530L |
|
| Chemical compound, drug |
Texas Red DHPE |
Thermo Fisher |
T1395MP |
|
| Recombinant protein |
Annexin V 647 |
Thermo Fisher |
A13204 |
|
| Chemical compound, drug |
Small molecule library: LOPAC-1280 (1278 compounds, 96-well) |
Sigma-Aldrich |
LO1280-1KT |
|
| Chemical compound, drug |
Small molecule library: LOPAC-Pfizer (90 Pfizer-drugs, 96-well) |
Sigma-Aldrich |
LO5100 |
|
| Chemical compound, drug |
Small molecule library: L1200 (355 kinase inhibitors, 96-well) |
Selleck Chemicals |
L1200 |
|
| Chemical compound, drug |
Small molecule library: L1900 (120 epigenetic compounds, 96-well) |
Selleck Chemicals |
L1900 |
|
| Chemical compound, drug |
Small molecule library: L2300 (378 cancer compounds, 96-well) |
Selleck Chemicals |
L2300 |
|
| Chemical compound, drug |
Small molecule library: L4200 (1364 FDA-approved drugs, 96-well) |
TargetMol |
L4200 |
|
| Chemical compound, drug |
Small molecule library: Spectrum collection (2400 bioactive compounds, 96-well) |
Microsource Discovery |
N/A |
|
| Chemical compound, drug |
Nelfinavir mesylate |
R and D Systems |
3766/10 |
|
| Chemical compound, drug |
Heparin |
Sigma-Aldrich |
H3393\ |
|
| Chemical compound, drug |
Nocodazole |
Sigma-Aldrich |
487928 |
|
| Chemical compound, drug |
Amiloride/EIPA |
Sigma-Aldrich |
A3085 |
|
| Chemical compound, drug |
Wortmannin |
Sigma-Aldrich |
W1628-1MG |
|
| Chemical compound, drug |
Chlorpromazine |
Sigma-Aldrich |
215921–500 MG |
|
| Chemical compound, drug |
Filipin |
Sigma-Aldrich |
F4767-1MG |
|
| Chemical compound, drug |
Nystatin |
Thermo Fisher |
BP29495 |
|
| Chemical compound, drug |
Leupeptin |
Sigma-Aldrich |
L2884-5MG |
|
| Chemical compound, drug |
AEBSF |
Sigma-Aldrich |
A8456 |
|
| Chemical compound, drug |
Furin inhibitor II (polyarginine) |
Sigma-Aldrich |
344931 |
|
| Chemical compound, drug |
Decanoyl-RVKR-CMK (chloromethylketone) |
Tocris |
3501 |
|
| Chemical compound, drug |
Methyl-Beta-cyclodextrin (MBCD) |
Sigma-Aldrich |
C4555-1G |
|
| Chemical compound, drug |
Lovastatin |
Sigma-Aldrich |
438185 |
|
| Chemical compound, drug |
Camostat mesylate |
Sigma-Aldrich |
SML0057 |
|
| Chemical compound, drug |
E-64D (EST) |
Sigma-Aldrich |
33000 |
|
| Chemical compound, drug |
Ammonium chloride |
Sigma-Aldrich |
A9434 |
|
| Chemical compound, drug |
Chloroquine diphosphate |
Sigma-Aldrich |
C6628 |
|
| Chemical compound, drug |
Gefitinib |
Sigma-Aldrich |
SML1657 |
|
| Chemical compound, drug |
BAPTA-AM |
Sigma-Aldrich |
196419 |
|
| Chemical compound, drug |
Latrunculin A |
Invitrogen |
428026 |
|
| Chemical compound, drug |
Rottlerin |
Sigma-Aldrich |
557370- |
|
| Chemical compound, drug |
Dynasore |
Sigma-Aldrich |
324410 |
|
| Chemical compound, drug |
Sodium chlorate |
Sigma-Aldrich |
403016 |
|
| Chemical compound, drug |
Protease inhibitor cocktail |
Sigma-Aldrich |
P1860-1ML |
|
| Chemical compound, drug |
Ouabain |
Sigma-Aldrich |
4995–1 GM |
|
| Chemical compound, drug |
Rostafuroxin/PST2238 |
Sigma-Aldrich |
SML1139 |
|
| Chemical compound, drug |
Silmitasertib |
Selleck Chemicals |
S2248 |
|
| Chemical compound, drug |
R-406 |
Selleck Chemicals |
.S2194 |
|
| Chemical compound, drug |
Apilimod |
Selleck Chemicals |
S6414 |
|
| Chemical compound, drug |
Bafilomycin A |
Enzo |
BML-CM110 |
|
| Chemical compound, drug |
2-Bromopalmitic acid (2 BP) |
Sigma-Aldrich |
238422–10G |
|
| Chemical compound, drug |
MG132 |
Sigma-Aldrich |
M8699 |
|
| Chemical compound, drug |
Monensin |
Sigma-Aldrich |
M5273 |
|
| Chemical compound, drug |
Brefeldin A |
Sigma-Aldrich |
B5936 |
|
| Chemical compound, drug |
Cycloheximide |
Sigma-Aldrich |
C4859-1ML |
|
| Chemical compound, drug |
Dimethyl sulfoxide (DMSO) |
Sigma-Aldrich |
D2650 |
|
| Chemical compound, drug |
Actinomycin D |
Sigma-Aldrich |
A5156-1VL |
|
| Chemical compound, drug |
Staurosporine |
AbCam |
ab120056 |
|
| Chemical compound, drug |
ISRIB |
Sigma-Aldrich |
SML0843 |
|
| Chemical compound, drug |
3-Methyladenine |
Sigma-Aldrich |
M9281 |
|
| Chemical compound, drug |
Z-FA-FMK |
Selleck Chemicals |
S7391 |
|
| Chemical compound, drug |
NSC 23766 |
Selleck Chemicals |
S8031 |
|
| Chemical compound, drug |
Z-VAD-FMK |
Selleck Chemicals |
S7023 |
|
| Chemical compound, drug |
AZD8055 |
Selleck Chemicals |
S1555 |
|
| Chemical compound, drug |
25-Hydroxycholesterol |
Sigma-Aldrich |
H1015 |
|
| Chemical compound, drug |
Aprotinin (BPTI) |
Selleck Chemicals |
.S7377 |
|
| Chemical compound, drug |
Genistein |
Selleck Chemicals |
S1342 |
|
| Chemical compound, drug |
Oleic acid (MBCD-conjugated) |
Sigma-Aldrich |
O1257 |
|
| Chemical compound, drug |
Cholesterol (MBCD-conjugated) |
Sigma-Aldrich |
C4951 |
|
| Chemical compound, drug |
Linoleic acid (MBCD-conjugated) |
Sigma-Aldrich |
L5900 |
|
| Chemical compound, drug |
Myriocin |
Sigma-Aldrich |
M1177-5MG |
|
| Chemical compound, drug |
Zaragozic Acid A |
Cayman |
17452 |
|
| Chemical compound, drug |
Digitonin |
Sigma-Aldrich |
D141 |
|
| Chemical compound, drug |
Bortezomib |
Selleck Chemicals |
S1013 |
|
| Chemical compound, drug |
Bupivacaine hydrochloride |
Sigma-Aldrich |
PHR1128 |
|
| Chemical compound, drug |
Ropivacaine hydrochloride |
Selleck Chemicals |
S4058 |
|
| Chemical compound, drug |
Naftifine hydrochloride |
Cayman |
19234 |
|
| Chemical compound, drug |
Terbinafine hydrochloride |
Cayman |
10011619 |
|
| Chemical compound, drug |
Propofol |
Sigma-Aldrich |
P-076–1 ML |
|
| Chemical compound, drug |
Puromycin |
Sigma-Aldrich |
P7255 |
|
| Chemical compound, drug |
Blasticidin S |
VWR |
1859–25 |
|
| Chemical compound, drug |
DAPI |
Sigma-Aldrich |
D9542 |
|
| Chemical compound, drug |
Hoechst 33342 Solution |
Thermo Fisher |
62249 |
|
| Chemical compound, drug |
AMG 9810 |
Cayman |
14715 |
|
| Chemical compound, drug |
Apomorphine hydrochloride |
ApexBio |
B6936 |
|
| Chemical compound, drug |
Avanafil |
Cayman |
23024 |
|
| Chemical compound, drug |
BML-277 |
Cayman |
17552 |
|
| Chemical compound, drug |
Canagliflozin |
Cayman |
11575 |
|
| Chemical compound, drug |
Chlorohexidine |
Targetmol |
T1147 |
|
| Chemical compound, drug |
CP-471474 |
Cayman |
29442 |
|
| Chemical compound, drug |
Gossypol |
ApexBio |
N2135 |
|
| Chemical compound, drug |
Indacaterol maleate |
ApexBio |
B1369 |
|
| Chemical compound, drug |
JFD00244 |
Cayman |
14648 |
|
| Chemical compound, drug |
Miconazole nitrate |
Cayman |
15420 |
|
| Chemical compound, drug |
Moxidectin |
ApexBio |
B3611 |
|
| Chemical compound, drug |
PD-407824 |
Cayman |
25989 |
|
| Chemical compound, drug |
Rapamycin |
Targetmol |
T1537 |
|
| Chemical compound, drug |
Ritanserin |
Tocris |
1955 |
|
| Chemical compound, drug |
Rolapitant hydrochloride |
Targetmol |
T3724 |
|
| Chemical compound, drug |
Scriptaid |
ApexBio |
A4106 |
|
| Chemical compound, drug |
SKI II |
Cayman |
10009222 |
|
| Chemical compound, drug |
Sorafenib |
Cayman |
10009644 |
|
| Chemical compound, drug |
TAK-285 |
MCE |
HY-15196 |
|
| Chemical compound, drug |
Ticagrelor |
Cayman |
15425 |
|
| Chemical compound, drug |
TW-37 |
Cayman |
20999 |
|
| Chemical compound, drug |
UK-356618 |
ApexBio |
A4440 |
|
| Chemical compound, drug |
Vorapaxar |
ApexBio |
A8809 |
|
| Chemical compound, drug |
CholEsteryl BODIPY (FL C12) |
Thermo Fisher |
C3927MP |
|
| Chemical compound, drug |
Fibronectin from bovine plasma |
Sigma-Aldrich |
F1141-5MG |
|
| Cell line (Homo sapiens, female) |
HEK293T |
Marc Diamond, UTSW |
N/A |
|
| Cell line (H. sapiens, female) |
U2OS |
Tom Muir, Princeton |
N/A |
|
| Cell line (H. sapiens, male) |
Beas2B |
Celeste Nelson, Princeton |
N/A |
|
| Cell line (H. sapien, males) |
A549 (ACE2/TMPRSS2) |
This study |
N/A |
|
| Cell line (H. sapiens, male) |
Calu3 |
ATCC |
HTB-55 |
|
| Cell line (Cercopithecus aethiops, female) |
VeroE6 (monkey) |
ATCC |
CRL-1586 |
|
| Cell line (H. sapiens, female) |
U2OS, various |
This study |
N/A |
|
| Cell line (C. aethiops, female) |
VeroE6 (monkey), various |
This study |
N/A |
|
| Recombinant DNA reagent |
SCARB1-GFP |
Addgene |
#86979 |
|
| Recombinant DNA reagent |
GFP1-10 |
Addgene |
#80409 |
|
| Recombinant DNA reagent |
GFP11 × 7-mCherry-a-tubulin |
Addgene |
#70218 |
|
| Recombinant DNA reagent |
LAMP1-mCherry |
Addgene |
#45147 |
|
| Recombinant DNA reagent |
mCherry-CAV1 |
Addgene |
#55008 |
|
| Recombinant DNA reagent |
GFP-EEA1 |
Addgene |
#42307 |
|
| Recombinant DNA reagent |
SARS-CoV-1-Spike |
Addgene |
#145031 |
|
| Recombinant DNA reagent |
RAC1 (H. sapiens) in pANT7_cGST |
DNASU |
CD00632727 |
|
| Recombinant DNA reagent |
ACE2 cDNA in pcDNA3.1 |
Genscript |
OHu20260 |
|
| Recombinant DNA reagent |
SARS-CoV-2 (2019-nCoV) Spike ORF (Codon Optimized) |
Sino Biological |
VG40589-UT |
|
| Recombinant DNA reagent |
pCAGGS-SARS-CoV2-S |
BEI Resources, NIH |
N/A |
|
| Recombinant DNA reagent |
pMLV gag-pol |
Ploss Lab, Princeton |
N/A |
|
| Recombinant DNA reagent |
pLMN8-Gluc |
This study |
N/A |
|
| Recombinant DNA reagent |
p-LAT Transmembrane-mRFP |
This study |
N/A |
|
| Recombinant DNA reagent |
PSP (lentivirus packaging plasmid) |
Marc Diamond, UTSW |
N/A |
|
| Recombinant DNA reagent |
VSVG (lentivirus packaging plasmid) |
Marc Diamond, UTSW |
N/A |
|
| Recombinant DNA reagent |
FM5 lentiviral vector (Ubiquitin C promoter) |
Sanders et al., 2014 |
N/A |
|
| Recombinant DNA reagent |
FM5-mGFP-Standardized glycine-serine (GS) Linker-AscI Site-STOP |
Sanders et al., 2020 |
N/A |
|
| Recombinant DNA reagent |
FM5-mCherry-Standardized GS Linker-AscI Site-STOP |
Sanders et al., 2020 |
N/A |
|
| Recombinant DNA reagent |
FM5-miRFP670-Standardized GS Linker-AscI Site-STOP |
Sanders et al., 2020 |
N/A |
|
| Recombinant DNA reagent |
FM5-NheI Site-Standardized GS Linker-mGFP |
Sanders et al., 2020 |
N/A |
|
| Recombinant DNA reagent |
FM5-NheI Site-Standardized GS Linker-mCherry |
Sanders et al., 2020 |
N/A |
|
| Recombinant DNA reagent |
FM5-NheI Site-Standardized GS Linker-miRFP670 |
Sanders et al., 2020 |
N/A |
|
| Recombinant DNA reagent |
FM5-NheI Site-Standardized GS Linker-EYFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-HNRNPA1-EYFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-FUS-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-GFP1-10 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-GFP11 × 7 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-mGFP-EEA1 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-miRFP670-EEA1 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-mGFP-RAC1 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-miRFP670-RAC1 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-mGFP-CAV1 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-miRFP670-CAV1 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-LAMP1-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-LAMP1- miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-SCARB1-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-SCARB1-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-MYMK-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-MYMK-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-MYMK-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-MYMX-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-MYMX-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-MYMX-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-p14 (Reptilian orthoreovirus)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-p14 (Reptilian orthoreovirus)-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-p14 (Reptilian orthoreovirus)- miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Albumin signal sequence (SS)−8 amino acid GS Linker-B7 Transmembrane (TM)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Albumin SS-8 amino acid GS Linker-B7 TM-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Albumin SS-8 amino acid GS Linker-B7 TM-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-ACE2-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-ACE2-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-ACE2-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-ACE2 Ectodomain-8 amino acid GS Linker-B7 TM/CTD-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-ACE2 Ectodomain-8 amino acid GS Linker-B7 TM-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-ACE2 Ectodomain-8 amino acid GS Linker-B7 TM-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-SARS-CoV-1 Spike-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-SARS-CoV-1 Spike-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-SARS-CoV-1 Spike-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike Full-length (FL) (1–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike Full-length (FL) (1–1273)-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike Full-length (FL) (1–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-RBD (319-541)−8 amino acid GS Linker-B7 TM/CTD-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-RBD (319-541)−8 amino acid GS Linker-B7 TM/CTD-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-RBD (319-541)−8 amino acid GS Linker-B7 TM/CTD-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-RBD (319-541)-WT TM/CTD (1203–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-RBD (319-541)-WT TM/CTD (1203–1273)-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-RBD (319-541)-WT TM/CTD (1203–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike Ectodomain (1–1213)−8 amino acid GS Linker-B7 TM/CTD-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike Ectodomain (1–1213)−8 amino acid GS Linker-B7 TM/CTD-mCherry |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike Ectodomain (1–1213)−8 amino acid GS Linker-B7 TM/CTD-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike B7 TM Swap with Extracellular Linker (1-1213-8 amino acid GS linker-B7 TM-1235–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike B7 TM Swap with Extracellular Linker (1-1213-8 amino acid GS linker-B7 TM-1235–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike B7 TM Swap (1–1213-B7 TM-1235–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike B7 TM Swap (1–1213-B7 TM-1235–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ITGA1 TM Swap (1–1213-ITGA1 TM-1235–1273)--mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ITGA1 TM Swap (1–1213-ITGA1 TM-1235–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1268-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1268-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1266-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1266-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1263-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1263-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1260-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1260-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1256-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1256-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1245-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1245-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1239-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1239-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1234 (ΔCTD)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike 1–1234 (ΔCTD)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike CysAlaMutant (C1235A/C1236A/C1240A/C1241A/C1243A/C1247A/C1248A/C1250A/C1253A/C1254A)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike CysAlaMutant (C1235A/C1236A/C1240A/C1241A/C1243A/C1247A/C1248A/C1250A/C1253A/C1254A)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike C1235A/C1236A-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike C1235A/C1236A-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-ΔNTD (319–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-ΔNTD (319–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔRBD (1–318, 540–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔRBD (1–318, 540–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔFusionPeptide (1–787, 807–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔFusionPeptide (1–787, 807–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔHR1 (1–919, 971–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔHR1 (1–919, 971–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔHR2 (1–1162, 1203–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔHR2 (1–1162, 1203–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔBothCleavageSites (‘CS’) (Δ685/Δ686/Δ815/Δ816)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔBothCS (Δ685/Δ686/Δ815/Δ816)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔS1/S2CS (Δ685/Δ686)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔS1/S2CS (Δ685/Δ686)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔS2’CS (Δ815/Δ816)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike ΔS2’CS (Δ815/Δ816)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S813G-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S813G-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S816G-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S816G-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike R815A-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike R815A-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike R815K-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike R815K-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike R815A/S816G-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike R815A/S816G-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike R815K/S816G-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike R815K/S816G-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S813G/R815A-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S813G/R815A-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S813G/R815K-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S813G/R815K-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S1 (1–685)-WT TM/CTD (1203–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S1 (1–685)-WT TM/CTD (1203–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S1 (1–685)−8 amino acid GS Linker-B7 TM/CTD-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike S1 (1–685)−8 amino acid GS Linker-B7 TM/CTD-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-S2 (686–1273)-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-S2 (686–1273)-miRFP670 |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-S2 (686–1213)−8 amino acid GS Linker-B7 TM/CTD Swap-mGFP |
This study |
N/A |
|
| Recombinant DNA reagent |
FM5-Spike SS (1-12)-S2 (686–1213)−8 amino acid GS Linker B7 TM/CTD Swap-miRFP670 |
This study |
N/A |
|
| Software, algorithm |
ChemAxon Physicochemical Property Analysis |
Morgan et al., Angew. Chem. Int. Ed., 2018 |
https://chemaxon.com/ |
|
| Software, algorithm |
R Software Mann-Whitney U Statistical Analysis |
Morgan et al., Angew. Chem. Int. Ed., 2018 |
https://www.r-project.org/ |
|
| Software, algorithm |
NCGC Scaffold Hopper |
NCATS Chemical Genomics Centre (NCGC) |
https://tripod.nih.gov |
|
| Software, algorithm |
RDKit Substructure Analysis |
RDKit |
https://www.rdkit.org/ |
|
| Software, algorithm |
Python Software |
Python |
https://www.python.org/ |
|
| Software, algorithm |
GraphPad Prism Nonlinear Regression |
GraphPad |
https://www.graphpad.com/ |
|
| Software, algorithm |
MATLAB R2017b |
Mathworks |
https://www.mathworks.com/ |
|
| Software, algorithm |
ImageJ |
Schneider et al., Nature Methods, 2012 |
https://imagej.nih.gov/ij/ |
|
| Software, algorithm |
PANTHER v14.0 Gene Ontology |
Mi et al., Nature Protocols, 2019 |
https://pantherdb.org |
|
| Software, algorithm |
BioRender (Paid Academic Plan) |
BioRender |
https://bio-render.com |
|
