| Gene (Homo sapiens) |
FOXM1 |
GenBank |
Gene ID: 2305 |
|
| Gene (Homo sapiens) |
RHNO1 |
GenBank |
Gene ID: 83695 |
|
| Antibody |
FOXM1 (rabbit monoclonal) |
Cell Signaling Technology |
5436 |
(1:1000) |
| Antibody |
β-Actin (mouse monoclonal) |
Santa Cruz |
sc-47778 |
(1:200) |
| Antibody |
α-Tubulin (rabbit polyclonal) |
Cell Signaling Technology |
2144 |
(1:1000) |
| Antibody |
Lamin B (goat polyclonal) |
Santa Cruz |
sc-6217 |
Discontinued |
| Antibody |
Histone H3 (goat polyclonal) |
Santa Cruz |
sc-8654 |
Discontinued |
| Antibody |
CHEK1 (mouse monoclonal) |
Santa Cruz |
sc-8408 |
(1:200) |
| Antibody |
P-CHEK1 S345 (rabbit monoclonal) |
Cell Signaling Technology |
2348 |
(1:1000) |
| Antibody |
P-CHEK1 S317 (rabbit monoclonal) |
Cell Signaling Technology |
12302 |
(1:1000) |
| Antibody |
RPA32/RPA2 (rabbit monoclonal) |
Abcam |
ab76420 |
(1:1000) |
| Antibody |
RPA32 (S33) (rabbit polyclonal) |
Bethyl |
A300-246A-M |
(1:1000) |
| Antibody |
H2AX (goat polyclonal) |
Bethyl |
A303-837A |
(1:2000) |
| Antibody |
P-H2AX S139 (rabbit monoclonal) |
Cell Signaling Technology |
9718 |
(1:1000) |
| Antibody |
Flag (rabbit monoclonal) |
Cell Signaling Technology |
14793 |
(1:1000) |
| Antibody |
HA (rabbit monoclonal) |
Cell Signaling Technology |
3724 |
(1:1000) |
| Antibody |
RHNO1 (rabbit polyclonal) |
Novus |
NBP1-93694 |
Discontinued |
| Antibody |
RHNO1 (rabbit polyclonal) |
Sigma |
HPA038682 |
Discontinued |
| Antibody |
TOPBP1 (rabbit monoclonal) |
Cell Signaling Technology |
14342 |
(1:1000) |
| Antibody |
RAD9 (rabbit polyclonal) |
Santa Cruz |
sc-8324 |
Discontinued |
| Antibody |
RAD1 (rabbit monoclonal) |
Invitrogen |
702149 |
(1–2 µg/mL) |
| Antibody |
HUS1 (rabbit monoclonal) |
Cell Signaling Technology |
16416 |
(1:1000) |
| Antibody |
Anti-HA magnetic beads (mouse monoclonal) |
Pierce |
88837 |
|
| Cell line (Homo sapiens) |
COV362 |
Sigma |
7071904 |
|
| Cell line (Homo sapiens) |
COV318 |
Sigma |
7071903 |
|
| Cell line (Homo sapiens) |
KURAMOCHI |
JCRB |
JCRB0098 |
|
| Cell line (Homo sapiens) |
OVSAHO |
JCRB |
JCRB1046 |
|
| Cell line (Homo sapiens) |
SNU-119 |
Korean Cell Line Bank |
00119 |
|
| Cell line (Homo sapiens) |
OVCAR4 |
NCI |
|
|
| Cell line (Homo sapiens) |
OVCAR8 |
NCI |
|
|
| Cell line (Homo sapiens) |
OVCAR3 |
ATCC |
HTB-161 |
|
| Cell line (Homo sapiens) |
CAOV3 |
Anirban Mitra |
|
|
| Cell line (Homo sapiens) |
OVCAR5 |
Anirban Mitra |
|
|
| Cell line (Homo sapiens) |
UWB1.289 |
Lee Zou |
|
|
| Cell line (Homo sapiens) |
UWB1-SyR12 |
Lee Zou |
|
|
| Cell line (Homo sapiens) |
UWB1-SyR13 |
Lee Zou |
|
|
| Cell line (Homo sapiens) |
hOSE |
ScienCell |
7310 |
|
| Cell line (Homo sapiens) |
FT190 |
Ronny Drapkin |
|
|
| Cell line (Homo sapiens) |
FT282-E1 |
Ronny Drapkin |
|
|
| Cell line (Homo sapiens) |
FT282 |
Ronny Drapkin |
|
|
| Cell line (Homo sapiens) |
IOSE-21 |
Francis Balkwill |
|
|
| Cell line (Homo sapiens) |
IOSE-121 |
Nelly Auersperg |
|
|
| Cell line (Homo sapiens) |
283T |
ATCC |
CRL-3216 |
|
| Cell line (Homo sapiens) |
U2OS-DR-GFP (282C) |
Jeremy Stark |
|
|
| Cell line (Homo sapiens) |
OVCAR8-DR-GFP |
Larry Karnitz and Scott Kaufmann |
|
|
| Chemical compound, drug |
Berzosertib |
SelleckChem |
VE-822 |
|
| Chemical compound, drug |
Olaparib |
SelleckChem |
ABT-888 |
|
| Chemical compound, drug |
Hydroxyurea |
Sigma |
H8627 |
|
| Chemical compound, drug |
Carboplatin |
Sigma |
C2538 |
|
| Chemical compound, drug |
Etoposide |
Sigma |
E1383 |
|
| Chemical compound, drug |
Doxycycline |
Sigma |
D9891 |
|
| Commercial assay or kit |
Direct-zol RNA Purification Kit |
Zymo Research |
R2072 |
|
| Commercial assay or kit |
Comet Assay Kit |
Trevigen |
4250-050-K |
|
| Commercial assay or kit |
iScript cDNA Synthesis Kit |
Bio-Rad |
1708890 |
|
| Commercial assay or kit |
DNA-free kit |
Ambion |
AM1906 |
|
| Commercial assay or kit |
NE-PER Nuclear and Cytoplasmic Extraction Kit |
Pierce |
78833 |
|
| Commercial assay or kit |
BCA protein assay |
Pierce |
23225 |
|
| Commercial assay or kit |
Enhanced chemiluminescence |
Pierce |
32106 |
|
| Commercial assay or kit |
Puregene Tissue Kit |
QIAGEN |
158667 |
|
| Commercial assay or kit |
EZ DNA Methylation Kit |
Zymo Research |
D5001 |
|
| Commercial assay or kit |
QIAquick Gel Extraction Kit |
QIAGEN |
28704 |
|
| Commercial assay or kit |
TOPO TA Cloning Kit |
Invirogen |
K457501 |
|
| Commercial assay or kit |
FirstChoice RLM-RACE Kit |
Ambion |
AM1700 |
|
| Commercial assay or kit |
Dual-Luciferase Reporter Assay System |
Promega |
E1910 |
|
| Commercial assay or kit |
Phospha-Light SEAP Reporter Gene Assay System |
Thermo Scientific |
T1015 |
|
| Commercial assay or kit |
TRIzol reagent |
Thermo Scientific |
15596026 |
|
| Commercial assay or kit |
Lipofectamine 2000 reagent |
Life Technologies |
11668019 |
|
| Commercial assay or kit |
QuickExtract |
Lucigen |
QE09050 |
|
| Commercial assay or kit |
M-PER |
Pierce |
78501 |
|
| Commercial assay or kit |
Halt Protease and Phosphatase Cocktail |
Pierce |
78440 |
|
| Commercial assay or kit |
Turbo Nuclease |
Sigma |
T4330-50KU |
|
| Commercial assay or kit |
AlamarBlue |
Bio-Rad |
BUF012A |
|
| Commercial assay or kit |
Vectashield with DAPI |
Vector Laboratories |
H-1200-10 |
|
| Genetic reagent (Homo sapiens) |
pCBASceI |
Addgene |
26477 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pCW57-GFP-P2A-MCS |
Addgene |
71783 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pCW57-GFP-P2A-MCS-(Neo) |
Addgene |
89181 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pCW57-MCS1-P2A-MCS2-(Hygro) |
Addgene |
80922 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pCW57-MCS1-P2A-MCS2-(Blast) |
Addgene |
80921 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pCW57-RFP-P2A-MCS |
Addgene |
78933 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pCW57-RFP-P2A-MCS-(Neo) |
Addgene |
89182 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pTRIPZ Non-silencing |
Dharmacon |
NC0257175 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pGL4 Luc Rluc Empty |
Addgene |
64034 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pCMV6-SEAP |
Addgene |
24595 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pTurbo-GFP-RFP-empty |
Custom |
|
Plasmid |
| Genetic reagent (Homo sapiens) |
pTurbo-GFP-PGK |
Custom |
|
Plasmid |
| Genetic reagent (Homo sapiens) |
pTurbo-RFP-PGK |
Custom |
|
Plasmid |
| Genetic reagent (Homo sapiens) |
pTurboGFP-RFP-FOXM1-RHNO1 |
Custom |
|
Plasmid |
| Genetic reagent (Homo sapiens) |
pLentiCRISPRv2 |
Addgene |
52961 |
Plasmid |
| Genetic reagent (Homo sapiens) |
PX458-WT-Cas9-empty |
Addgene |
48138 |
Plasmid |
| Genetic reagent (Homo sapiens) |
lenti-SAMv2-Puro |
Addgene |
92062 |
Plasmid |
| Genetic reagent (Homo sapiens) |
lenti-MPHv2-Neo |
Addgene |
92065 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2A-Puro (KRAB) |
Addgene |
71236 |
Plasmid |
| Genetic reagent (Homo sapiens) |
psPAX2 |
Addgene |
12260 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pMD2.G |
Addgene |
12259 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pOTB7-RHNO1 |
Harvard PlasmID Repository |
HsCD00326310 |
Plasmid |
| Genetic reagent (Homo sapiens) |
pENTR-MGC RHNO1 siR |
Stephen Elledge |
|
Plasmid |
| Genetic reagent (Homo sapiens) |
pENTR-MGC RHNO1 SWV |
Stephen Elledge |
|
Plasmid |
| Genetic reagent (Homo sapiens) |
pCMV6 AN-HA empty |
Origene |
PS100013 |
Plasmid |
| Genetic reagent (Homo sapiens) |
AdNGUS24i |
Frank Graham and Phillip Ng |
|
Adenovirus |
| Software, algorithm |
Prism 8 statistical software |
GraphPad |
|
|
| Software, algorithm |
Lasergene SeqMan Pro |
DNASTAR |
|
|
| Software, algorithm |
Comet Analysis Software |
Trevigen |
|
|
| Software, algorithm |
R package |
R Project |
|
|
| Software, algorithm |
Cell Ranger Single Cell Software |
10X Genomics |
|
|
| Software, algorithm |
GSEA software version 3 |
Broad Institute |
|
|
| Software, algorithm |
Cufflinks (v2.1.1) |
https://doi.org/10.1038/nbt.1621 |
|
|
| Software, algorithm |
Trim Galore software package |
The Babraham Institute |
|
|
| Software, algorithm |
TopHat (v2.0.8) |
https://doi.org/10.1186/gb-2013-14-4-r36 |
|
|
| Software, algorithm |
Cuffdiff (v2.1.1) |
https://doi.org/10.1038/nbt.2450 |
|
|
| Software, algorithm |
Perl |
The Perl Foundation |
|
|
| Software, algorithm |
scImpute |
https://doi.org/10.1038/s41467-018-03405-7 |
|
|
