TABLE 1.
Francisella strains and constructs used in the study
| Strain/construct | Subspecies | Description | Source and/or reference |
|---|---|---|---|
| U112 | F. novicida | Identified as FSC040 here and in previous publications | Water, 1950, Utah (1) |
| FSC200 | F. tularensis subsp. holarctica | Wild-type strain | Human ulcer, 1998, Sweden (76) |
| LVS | F. tularensis subsp. holarctica | Live vaccine strain; identified as FSC155 here and in previous publications | USAMRIID |
| Schu S4 | F. tularensis subsp. tularensis | Wild type strain. Identified as FSC237 here and in previous publications | Human ulcer, 1941, Ohio (77) |
| Schu S4 ΔwbtI | F. tularensis subsp. tularensis | Mutant of FSC237 characterized by loss of the long-chain O-polysaccharide | 47 |
| LVSpKK289Kan-gfp | F. tularensis subsp. holarctica | FSC155 expressing GFP in trans | This study |
| U112pKK289Kan-gfp | F. novicida | FSC040 expressing GFP in trans | This study |
| pKK289Kan-gfp | groELS promoter; Ft ori; p15A ori; GFP; Kanr | 71 |