FIG 3.

Characterization of ispS⁺ strains grown on trimethylamine (TMA) or acetate substrates. (a and e) Dimethylallyl pyrophosphate pyrophosphatase activity in cell extracts. (b and f) Endpoint methane production. (c and g) Isoprene production measured by gas chromatography. (d and h) Growth curves of att:pNB730 and att:ispS strains. Blue bars, att:pNB730 strain; red bars, att:ispS strain. Data presented in panels a to c and e to g were obtained from quadruplicate biological and triplicate technical replicates (n = 12). Data in panels d and h were from five biological replicates.