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Demonstration of isoprene production by Methanosarcina barkeri. (a) Endpoint methane assays for M. barkeri att:pNB730 and att:ispS strains. (b) Isoprene production by M. barkeri att:pNB730 and att:ispS strains as measured by gas chromatography. Blue, att:pNB730 strains; red, att:ispS strains. Data for panels a and b were obtained from quadruplicate biological and triplicate technical replicates (n = 12).
