TABLE 1.
Comparison of isoprene and terpenoid yields in engineered bacteria and archaea
| Organism | Yield (μmol mol−1 g−1) | Yield (μmol liter−1) | Condition | Citation |
|---|---|---|---|---|
| Batch culture yieldsa | ||||
| Clostridium ljungdahliib | 0.000147 | 0.21 | MES-fructose, batch | (60) |
| Synechocyctisb | 4.93 | not reported | Light + CO2, batch | (61) |
| Methanococcus maripaludisc | 41.1 | not reported | H2 + CO2, batch (geraniol) | (39) |
| Methanococcus maripaludisc | 67.53 | not reported | Formate, batch (geraniol) | (39) |
| Methanosarcina acetivorans | 886.67 | 855.01 | Methanol, batch | This study |
| Fed-batch yields | ||||
| Escherichia coli | not reported | 352,319.4 | 0.1–2% glucose, fed-batch | (45) |
| Saccharomyces cerevisiaed | not reported | 174,691.7 | 25 g liter−1 initial glucose, fed-batch | (46) |
a
Batch culture yields were reported in mg isoprene per mol substrate per g dry cell weight (DCW) or mg isoprene per liter.
b
Strains contain two or more mutations to enhance isoprene production.
c
Values are for the terpene geraniol.
d
The rate of feed solution and final concentration of glucose utilized were not reported.