TABLE 3.
Relative transcript abundance between att:ispS and att:pNB730 strains of M. acetivoransa
| Pathway | Gene | Fold change | P value |
|---|---|---|---|
| Mevalonate pathwayb | HMG-CoA synthase | 0.66 | 0.001271 |
| HMG-CoA reductase | 1.49 | 0.045242 | |
| Mevalonate kinase | 1.31 | 0.004854 | |
| Phosphomevalonate dehydratase | NT | NT | |
| Anhydromevalonate phosphate decarboxylase | NT | NT | |
| Isopentenyl phosphate kinase | 1.88 | 0.117332 | |
| Isopentenyl diphosphate delta-isomerase | 2.26 | 0.011509 | |
| TCA pathwayc | Pyruvate synthase | 0.80 | 0.211017 |
| Pyruvate carboxylase | 1.96 | 0.000206 | |
| Malate dehydrogenase | 1.68 | 0.007657 | |
| Fumarate hydratase | 0.21 | 1.53E−05 | |
| Methanogenesis | Methyl coenzyme M reductase B (mcrB) | 1.55 | 0.000121 |
aValues were obtained from triplicate biological replicates and 5 technical replicates (n = 15); NT, not tested.
bThe proposed archaeal mevalonate pathway in M. acetivorans differs from the eukaryotic pathway in the conversion of mevalonate to isopentenyl phosphate (19–21).
cAll annotated TCA cycle enzymes in M. acetivorans. Methanogens have an incomplete TCA cycle.