TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Feature(s)a | Source |
|---|---|---|
| Strains | ||
| E. coli DH5α | Host to replicate plasmids | TransGen |
| E. coli WM3064 | Host strain for conjugation; auxotroph | Lab stock |
| Pseudomonas sp. strain JY-Q | Wild type, able to degrade nicotine | Lab stock |
| Δspm1 | spm1 deleted in JY-Q | This study |
| Δspm2 | spm2 deleted in JY-Q | This study |
| Δspm1 Δspm2 | spm2 deleted from JY-Q Δspm1 strain | This study |
| ΔsirA2 | sirA2 deleted in JY-Q | This study |
| Δald1 (ΔnicB) | ald1 (nicB) deleted in JY-Q | This study |
| Δald2 | ald2 deleted in JY-Q | This study |
| Pseudomonas putida S-1 | Wild type, unable to degrade NA | Lab stock |
| S-1/pBBR | pBBR1MCS5 in S-1 | This study |
| S-1/pBBR-nicAB | pBBR1MCS5-nicAB in S-1 | This study |
| S-1/pBBR-ald2 | pBBR1MCS5-ald2 in S-1 | This study |
| Plasmids | ||
| pK18mobsacB | Suicide vector, mob+, sacB, Kmr | Lab stock |
| pK18mobsacB-spm1UD | pk18mobsacB with up- and downstream fragments of spm1 | This study |
| pK18mobsacB-spm2UD | pk18mobsacB with up- and downstream fragments of spm2 | This study |
| pK18mobsacB-sirA2UD | pk18mobsacB with up- and downstream fragments of sirA2 | This study |
| pK18mobsacB-ald1UD | pk18mobsacB with up- and downstream fragments of ald1 | This study |
| pK18mobsacB-ald2UD | pk18mobsacB with up- and downstream fragments of ald2 | This study |
| pBBR1MCS5 | Expression plasmid, Gmr | Lab stock |
| pBBR1MCS5-nicAB | pBBR1MCS5 containing nicAB | This study |
| pBBR1MCS5-ald2 | pBBR1MCS5 containing ald2 | This study |
a
Km, kanamycin; Gm, gentamycin; r, resistance.