FIG 2.
LitR is required for transformation of V. fischeri. Shown is the transformation frequency (total Trimr CFU/viable CFU) of V. fischeri cells exposed to gDNA from KV8300 (fliQ::Trim). (A) Transformation frequencies of plostfoX-Cm- or plostfoX-Kan-containing ES114 (WT) and litR strains PMF8 (litR::Kan) and JB19 (litR::Erm) containing plostfoX-Cm and plostfoX-Kan, respectively. (B) Transformation frequencies of ES114 and JB19 containing plostfoX-Kan and either vector control (pVSV105) or litR expression plasmid plitR (pPMF5). Both litR mutants PMF8 and JB19 exhibited transformation frequencies below the limit of detection (LOD). *, P < 0.05; **, P < 0.005; ns, not significant.