FIG 5.
A shift of the nonheme iron atom of NagGH, in comparison with NDO9816-4. (A and B) The superposition of the nonheme iron-binding motifs as well as some substrate-binding residues. NagGH and NDO9816-4 are shown in orange and gray, respectively. The shifts of residue Asp370 and the iron atom are indicated by dash lines. The close distance between Asp362 in NDO9816-4 and Leu228 in NagG is indicated as “clash.” (C) Sequence alignment of NagG and its homologs. NagG, AAD12607.1; 5AEU, WP_011494299.1; 1ULI, WP_011599002.1; 3GZX, Q46372.1; 1WQL, WP_039594666; 3EN1, WP_012052601.1; 4HJL, WP_011117400.1; 1NDO, WP_011117400.1; 2B1X, WP_087561958.1; 2BMO, AAL76202.1; 2GBW, WP_037508582.1; 5XBP, AGH09226.1; 2CKF, WP_037485720.1; 3N0Q, WP_011536837.1; 3VCA, WP_010967390.1. The sequence number of NagG is labeled on the top of the protein sequences. Green arrows indicate three residues potentially important for iron binding.