FIG 4.

Subcellular localization of FtsZ-GFP in filamentous Z6KF2 cells during recovery from stress. Growing Zm6 cells were subcultured in the complex medium supplemented with 0.2 M NaCl and grown for 5 h. The cells were then transferred to an agarose pad containing growth medium without additional salt. After 30 min of an incubation that allowed recovery and regrowth of the transferred cells, imaging commenced. Time-lapse imaging of two long filamentous cells is displayed. White arrowheads indicate the positions where cell division was completing or just completed. Note that positioning of FtsZ-GFP was not tightly controlled in the filamentous Zm6 cells. (A) A ladder-like FtsZ-GFP structure resulted in simultaneous divisions at 152 min. (B) Proximate double FtsZ-GFP rings, indicated by a yellow arrowhead, were observed. All images are overlays of phase-contrast and fluorescence images (green, FtsZ-GFP). Numbers indicate minutes after cells were mounted on the complex-medium–agarose pad. Bars, 5 μm.