TABLE 1.
Bacterial strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Description or sequence (5′-3′) | Source or reference |
|---|---|---|
| Strains | ||
| Zymomonas mobilis | ||
| Zm6 | ATCC 29191 | Lab stock |
| Zm4 | ATCC 31821 | Lab stock |
| Z6KF1 | Zm6 carrying pZKF1 | This study |
| Z6KF2 | Zm6 carrying pZKF2 | This study |
| Escherichia coli K-12 MG1655 | ATCC 700926 | Lab stock |
| Plasmids | ||
| pBBR1MCS-2 | Kmr | 69 |
| pZKF1 | pBBR1-MCS2 harboring ftsZ gene with its native promoter | This study |
| pZKF2 | pBBR1-MCS2 harboring ftsZ-gfp fusion gene with ftsZ promoter | This study |
| Primers | ||
| ZftsZF | ATTTTATCTAGATGCGTCGGAACCCTTTGATC | |
| ZftsZR_ws | ATTTTAAAGCTTGGAATTACCCTGACGATTCA | |
| ZftsZR | ATTTTAAAGCTTGGGTGCCGATAAATTCGGAC | |
| GFPF | ATTTTAAAGCTTGTACCAAGTAAAGGAGAAG | |
| GFPR | ATTTTACTCGAGTTATTTGTATAGTTCATCCATGC | |