TABLE 2.
Primer sequences for novel Giardia duodenalis assemblage B (genomotyping) MLST
| Gene target | Primer name | Usage | Primer sequence (5′ → 3′) | Length | Estimated Ta (°C)a | Reference |
|---|---|---|---|---|---|---|
| KWX12433 (6-pgd) | KWX12433_118f | F1 | GGR ATT RTT GCG CAR TCR CTT CC | 23 | 58 | This study |
| KWX12433_157f | F2 | GAC TAT AGY TCR CCA ATA GGC | 21 | 56 | This study | |
| KWX12433_1184r | R2 | TTR TAT CTT GCA GKC AGC TGR CA | 23 | 58 | This study | |
| KWX12433_1641r | R1 | CAG AGA TGT TCG YYT ACG AAA C | 22 | 58 | This study | |
| KWX13158 (hypothetical) | KWX13158_339f | F1 | GGT TAC YTT TCT AGG TGA YAT ATA | 24 | 58 | This study |
| KWX13158_1820r | R1 | CTR CAR AAC GGW AGR CTC ARG TC | 23 | 56 | This study | |
| KWX13158_1784r | R2 | CCC GTG AAT ACR CAY AAG CTA T | 22 | 58 | This study | |
| KWX13158_426f | F2 | CAG RGT GCC AAA TCT TTA CRC | 21 | 56 | This study | |
| KWX15086 (phkg2) | KWX15086_1393f | F1 | CTT GAC CTY AAT GCM TTY CTY ATG A | 25 | 58 | This study |
| KWX15086_1548f | F2 | AAT CTG TCC YCT YGA GAT TGC T | 22 | 58 | This study | |
| KWX15086_2586r | R1 | GCT YTT GTT CTG YCC AAG GCT | 21 | 58 | This study | |
| KWX15086_2564r | R2 | TGA AGA GCC TCC GAG AAR TC | 20 | 58 | This study |
a
Ta, annealing temperature.