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Acquired resistance to severe ethanol stress required new protein synthesis. Yeast cells were pretreated with 6% (vol/vol) ethanol for 180 min with or without 200 μg/ml cycloheximide (CHX) and then exposed to 10% (vol/vol) ethanol stress for 180 min. Intracellular levels of insoluble proteins were assayed by the silver staining of SDS-polyacrylamide gels.
