FIG 3.

Cross-protection between ethanol (EtOH) stress and thermal stress. (A) Cells were pretreated with or without mild stress (6% ethanol for 180 min or 37°C for 60 min) and then exposed to severe stress (10% ethanol for 180 min or thermal stress at 42°C for 60 min). Intracellular levels of insoluble proteins were assayed. **, P < 0.01 by the Student t test. (B) The formation of Hsp104-GFP foci was monitored under each condition. Representative images are shown in the upper panel. The quantification of cells containing foci is shown in the lower panel. One hundred cells under each condition were examined, and experiments were repeated 3 times (300 cells in total were examined). Bar, 5 μm.