FIG 4.

A pretreatment with mild stress affected protein expression under severe ethanol (EtOH) stress. Sis1-FLAG, Ssa3-FLAG, Fes1-FLAG, Hsp42-FLAG, Hsp104, and Btn2-FLAG levels under stress conditions were assayed by Western blotting. Yeast cells were pretreated with or without mild stress (6% ethanol stress for 180 min or thermal stress at 37°C for 60 min) and then exposed to 10% (vol/vol) ethanol stress for 0, 60, 120, or 180 min. Ponceaus S staining was performed to confirm equal loading and the transfer of all proteins. Protein levels were normalized by Ponceau S staining, and data are expressed as the mean ± SD (n = 3).