FIG 6.

Aggregation of Lsg1 in response to severe ethanol (EtOH) stress. (A) Cells expressing Lsg1-mRFP and Hsp104-GFP were treated with thermal stress at 42°C for 60 min or 10% (vol/vol) ethanol stress for 180 min. Bar, 5 μm. (B and C) Cells expressing Lsg1-GFP were pretreated with or without mild stress (6% ethanol for 180 min or 37°C for 60 min) and then exposed to 10% (vol/vol) ethanol for 180 min. Representative images of wild-type cells under each condition are shown in panel B. The quantification of cells containing Lsg1-GFP foci in various mutants is shown in panel C. One hundred cells under each condition were examined, and experiments were repeated 3 times (300 cells in total were examined).