FIG 7.

Effects of MG132 on insoluble protein levels in pretreated cells. Pretreated cells (wild-type and pdr5Δ) with 6% (vol/vol) ethanol (EtOH) for 180 min or at 37°C for 60 min were exposed to 10% (vol/vol) ethanol for 180 min in the presence or absence of 100 μM MG132, a potent cell-permeative inhibitor of proteasomes. (A) The intracellular levels of insoluble proteins were analyzed. Representative images are shown in the upper panel and quantified data in the lower panel. Relative ratios were calculated from three independent experiments. Each value is expressed as the mean ± SD (n = 3). (B) The quantification of cells containing Lsg1-GFP foci under each condition is shown. One hundred cells under each condition were examined, and experiments were repeated 3 times (300 cells in total were examined).