FIG 8.

The generation of insoluble proteins was suppressed in pretreated cells. The intracellular levels of insoluble proteins (A) and Lsg1-GFP foci (B) were analyzed every 15 min for the first 60 min after exposure to severe ethanol (EtOH) stress (10% [vol/vol]). (A) The intracellular levels of insoluble proteins were analyzed. Representative images are shown in the upper panel and quantified data in the lower panel. Relative ratios were calculated from three independent experiments. Each value is expressed as the mean ± SD (n = 3). (B) The quantification of cells containing Lsg1-GFP foci under each condition is shown. One hundred cells under each condition were examined, and experiments were repeated 3 times (300 cells in total were examined).