Figure 3.

Overexpression of CADM3 mutant activates the unfolded protein response. (A) Dual reporter luciferase RT4 cells were transiently transfected with plasmids encoding the human wild‐type (WT) and mutant (Y172C) CADM3. Increased levels of firefly and nano luciferase were observed in the mutant CADM3. (B) Immunofluorescence of U2OS transfected cells shows co-localization of both wild-type and mutant CADM3 with the endoplasmic reticulum marker GPR78-BiP. (C) U2OS cells co-transfected with CADM3 and CADM4 show CADM3 co-localization with CADM4 at cell-cell contact sites.