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. 2021 Apr 29;2021:5535562. doi: 10.1155/2021/5535562

Figure 3.

Figure 3

Measurement of IL-1β (a), IL-6 (b), and IL-10 (c) serum levels using ELISA (n = 5/group). Measurements were performed 48 h after surgery in the sham, CLP, DX, and βS groups, and 72 h after surgery in the IMI and IMI+βS groups. All data are normalized to the total protein levels measured by BCA assay (mean ± SD of 0.205 ± 0.0001, 0.205 ± 0.0001, 0.204 ± 0.0001, 0.204 ± 0.0001, 0.204 ± 0.0001, and 0.205 ± 0.0003 pg/mL in the sham, CLP, DX, βS, IMI, and IMI+βS groups, respectively), and expressed as the mean ± SD. Data were analyzed by ANOVA, followed by Tukey's post hoc test for sham, CLP, DX, and βS comparison. The IMI and IMI+βS groups were analyzed separately using unpaired t-test. #Significant difference compared with the sham group (p < 0.0001). ∗Significant difference compared with the CLP group (p < 0.05). ∗∗Significant difference compared with the DX group (p < 0.01). ∗∗∗∗Significant difference compared with the CLP group (p < 0.0001). +++Significant difference compared with the IMI group (p < 0.001). βS: β-sitosterol; CLP: cecal ligation and puncture; DX: dexamethasone; IMI: imipenem.