Table 2.
Summary of validation parameters obtained from clavulanic acid calibration curves in ACN, plasma, and meat
| ACN | Plasma | Meat | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Linearity | |||||||||
| Concentration range (μg·L−1/μg·kg−1) | 10–2000 | 10–2000 | 50–2000 | ||||||
| Number of points | 6 | 6 | 7 | ||||||
| Correlation coefficient, R2 | 0.9929 | 0.9991 | 0.9918 | ||||||
| Standard deviation of the correlation coefficient | 0.2857 | 0.1998 | 0.2217 | ||||||
| Slope, m | 0.0064 | 0.0113 | 0.0036 | ||||||
| Standard deviation of the slope, Sm | 0.00059 | 0.00034 | 0.00008 | ||||||
| Intercept, b | −0.0089 | −0.1093 | 0.0060 | ||||||
| Standard deviation of the intercept, Sb | 0.0974 | 0.0587 | 0.0688 | ||||||
| LOD (μg·L−1/μg·kg−1) | 3.18 | 3.09 | 2.57 | ||||||
| LOQ (μg·L−1/μg·kg−1) | 10.48 | 10.21 | 8.47 | ||||||
| Accuracy | |||||||||
| Recovery (%) | 94.6 | 105.7 | 95.6 | ||||||
| Within-run precision | |||||||||
| Concentration level (μg·L−1/μg·kg−1) | 10 | 500 | 2000 | 10 | 500 | 2000 | 50 | 400 | 2000 |
| Calculated concentration (μg·L−1/μg·kg−1) | 7.71 | 513.73 | 2076.28 | 12.3 | 518.96 | 2088.70 | 45.58 | 399.18 | 1955.81 |
| CV (%) | 13.6 | 6.5 | 7.7 | 10.9 | 2.8 | 5.3 | 8.5 | 6.5 | 6.9 |