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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Dec 24;41(2):e82–e96. doi: 10.1161/ATVBAHA.120.315485

Figure 2.

Figure 2.

AIBP regulates OxLDL-induced autophagy. (A to D) BMDM isolated from WT and Apoa1bp−/− mice were incubated with PBS or 25 μg/ml OxLDL for 24 h. Cell lysates were immunoblotted with antibodies against LC3 and β-ACTIN, and band intensities were quantified. For imaging, cells were stained with an anti-LC3 antibody and DAPI. The numbers of LC3 puncta per cell were counted from three independent experiments. (E and F) Cells were pretreated with or without 100 nM Baf for 1h and then incubated with 25 μg/ml OxLDL for 24 h. Cell lysates were immunoblotted with indicated antibodies, and band intensities were quantified. Mean±SEM; N=3–5. *, p<0.05; **, p<0.005; ***, p<0.0005; ****; p<0.0001. Scale bar: 20 μm.