Figure 2. TSP-1 deletion inhibits leptin-induced increase in plaque area in ApoE^−/− mice.

Mice were treated as described in Figure legend 1. Aortic root serial sections were stained with H & E. Aortic root luminal area (in mm²) and total aortic root area (i.e. area enclosed by the internal elastic lamina, in mm²) were measured using Image J; difference between total root area and luminal area denotes plaque area. Shown are A) representative images for H & E-staining (4X magnification) and summary data for plaque area in B) male and C) female mice genotypes. n=4 mice/sex per study group, 3–5 sections per aortic root. Black dotted line marks region used for quantification. Values denote plaque area of aortic root per section in each study group, expressed as mean ± SEM; For C) log-transformed data used for statistical analyses. *p≤0.05; ***p<0.0005