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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Dec 17;41(2):e112–e127. doi: 10.1161/ATVBAHA.120.314962

Figure 3. TSP-1 deletion reduces leptin-induced collagen deposition in ApoE−/− mice.

Figure 3.

Mice were treated as described in Figure legend 1. Aortic root serial sections were stained with Masson’s trichrome followed by hematoxylin counterstaining. Total lesion area and MT-positive area within lesions (measured in sq. pixels) was quantified using Image J (4X magnification); collagen content is depicted as %MT positive area shown in natural log scale. Shown are A) representative images of MT-staining and summary data for %MT positive area in B) male and C) female mice (n=3–4 mice/sex per study group, 3–6 sections per aortic root). Dotted line depicts region used for quantification. Values denotes collagen content of aortic root per section in each study group; all values are expressed as mean ± SEM. **p<0.005; ****p<0.0001; NS: not significant.