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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Dec 17;41(2):e112–e127. doi: 10.1161/ATVBAHA.120.314962

Figure 5. TSP-1 deletion abrogates leptin-induced inflammatory cell infiltration in ApoE−/− mice.

Figure 5.

Mice were treated as described in Figure 1. Aortic root sections were subjected to immunohistochemistry using CD68 and CD45 antibodies. Total lesion area and positive staining area of lesions (in sq. pixels) was quantified in each section using Image J. Shown are representative images (10X magnification) and summary graphs for A and B) macrophage and C and D) leukocyte content (n=3–6 mice/sex in each study group; 3–7 sections/aortic root). Yellow dotted line marks region used for quantification. Shown are %CD68 or %CD45 positive area of total lesion per section in each study group; all values are expressed as mean ± SEM; ***p<0.0005; ****p<0.0001