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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Dec 17;41(2):e112–e127. doi: 10.1161/ATVBAHA.120.314962

Figure 6. TSP-1 deficiency blocks leptin-induced increase in smooth muscle cell and proliferative cell abundance in ApoE−/− mice.

Figure 6.

Mice were treated as described in Figure 1. Aortic root sections were used in double immunohistochemistry with α-SMA (SMC marker) and PCNA (proliferation marker) antibodies. A) Shown are representative images for α-SMA (green) and PCNA (red) staining; merge (yellow) shows α-SMA and PCNA co-localization; DAPI denotes nuclei staining (magnification 10X). White dotted line marks region used in quantification; arrows indicate PCNA-positive SMC (yellow). Summary data for B) SMC and C) proliferative cell content are shown (n=3–6 mice/sex in each study group; 3–7 sections/aortic root). Data represents %SMA or %PCNA positive area of total lesion per section in each study group; values are expressed as mean ± SEM; **p<0.005; ***p<0.0005; ****p<0.0001