Figure 7. TSP1 deletion attenuates leptin-induced vimentin and pCREB expression accompanied with augmented SM-MHC expression in ApoE−/− mice.

Mice were treated as in Figure 1. Aortic lysates were subjected to western blotting as outlined in Methods. Shown are A) representative western blot images and B) summary graphs for densitometric quantification of immunoblots in male mice; β-actin or α-tubulin used as loading control. Results are presented as fold of PBS-treated ApoE^−/−; values are expressed as mean ± SEM (PBS-treated ApoE^−/−: n=2; other groups: n=3–5). For A), each lane denotes an individual mouse. Please note, all lane images show proteins loaded and detected on a single immunoblot; however, lanes were re-arranged to improve clarity of presentation. *p≤0.05; ***p<0.0005