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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Dec 17;41(2):e112–e127. doi: 10.1161/ATVBAHA.120.314962

Figure 8. TSP-1 deletion inhibits leptin-induced increase in plaque area in ApoE−/− mice on western diet.

Figure 8.

Six weeks old male ApoE−/− and age-matched TSP-1−/−/ApoE−/− littermate mice were maintained on Western diet until 12 wks of age. Beginning at 9 wks, mice were treated with leptin or PBS (similar to Figure 1) for three weeks. Aortic root serial sections were stained with A) 0.5% ORO followed by hematoxylin counterstaining and C) H & E. Shown are representative images (4X magnification) and summary data for A and B) ORO-positive lipid burden and C and D) plaque area (in sq. mm). Dotted line marks region used for quantification. Data depicts lipid burden and plaque area of aortic root per section in each study group; values expressed as mean ± SEM (n=3–4 mice/study group; 3–7 sections/aortic root). For B) SQRT-transformed data used for statistical analyses. *p≤0.05; ***p<0.0005; ****p<0.0001