KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Phospho-pi3k Y199 | Cell Signaling Technology | Cat# 4228, RRID:AB_659940 |
| Phospho-Akt T308 | Cell Signaling Technology | Cat# 4056, RRID:AB_331163) |
| Phospho-Akt S473 | Cell Signaling Technology | Cat# 4060, RRID:AB_2315049 |
| Pan Akt | Cell Signaling Technology | Cat# 4691, RRID:AB_915783 |
| Akt1 | Cell Signaling Technology | Cat# 2938, RRID:AB_915788 |
| Phospho-mTOR S2448 | Cell Signaling Technology | Cat# 2971, RRID:AB_330970 |
| Phospho-mTOR S1261 | Diane Fingar (University of Michigan) | Acosta-Jaquez et al., 2009 |
| Total mTOR | Cell Signaling Technology | Cat# 2983, RRID:AB_2105622 |
| Phospho-ULK1 S757 | Cell Signaling Technology | Cat# 6888, RRID:AB_10829226) |
| Phospho-ULK1 S777 | Millipore | Cat# ABC213, RRID:AB_2861423 |
| Total ULK1 | Cell Signaling Technology | Cat# 8054, RRID:AB_11178668 |
| Phospho-p70s6k T421/S424 | Cell Signaling Technology | Cat# 9204, RRID:AB_2265913) |
| Phospho-p70s6k T389 | Cell Signaling Technology | Cat# 9234, RRID:AB_2269803 |
| Total p70s6k | Cell Signaling Technology | Cat# 2708, RRID:AB_390722 |
| Phospho-4e-bp1 T37/46 | Cell Signaling Technology | Cat# 2855, RRID:AB_560835 |
| Total 4e-bp1 | Cell Signaling Technology | Cat# 9644, RRID:AB_2097841 |
| Phospho-gsk3α S21 | Cell Signaling Technology | Cat# 9316, RRID:AB_659836 |
| p-gsk3ß S9 | Cell Signaling Technology | Cat# 5558, RRID:AB_10013750 |
| GAPDH | Santa Cruz Biotechnology | Cat# sc-47724, RRID:AB_627678 |
| Green fluorescent protein (GFP) | Aves Labs | Cat# GFP-1010, RRID:AB_2307313) |
| HA-Tag | Cell Signaling Technology | Cat# 3724, RRID:AB_1549585 |
| Actin | Thermo Fisher Scientific | Cat# MA5-15739, RRID:AB_10979409) |
| Bacterial and Virus Strains | ||
| HSV-dominant-negative Akt-GFP | This study | |
| HSV-wildtype AKT-GFP | This study | |
| Biological Samples | ||
| Tissue repository Array Collection (105 samples in total) | Stanley Medical Research Institute | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Alexa Fluor 488 | Jackson Immuno Research | Cat# 703-545-155, RRID:AB_2340375 |
| Lipofectamine 2000 | Invitrogen | Cat# 11668019 |
| Opti-MEM | Gibco | Cat# 31985062 |
| DL-AP5 (for cell culture) | Tocris | Cat# 0105 |
| Vectashield Hardset antifade mounting medium | Vector Laboratories | Cat# SKU: H-1400 |
| Cytosine B-D-arabinofuranoside | Sigma | Cat# C1768 |
| L-glutamine | Invitrogen | Cat# 25030081 |
| Sodium pyruvate | Invitrogen | Cat# 11360070 |
| Penicillin/streptomycin | Invitrogen | Cat# 15140122 |
| Critical Commercial Assays | ||
| RNeasy Plus Micro Kit | Qiagen | Cat# 74034 |
| High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor | Applied Biosystems | Cat# 4374966 |
| Picrotoxin | Sigma-Aldrich | Cat# P1675 |
| Tetrodotoxin citrate | Hello Bio | Cat# HB1035 |
| D-AP5 (for e-phys) | Hello Bio | Cat# HB0225 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| C57BL/6J | Jackson Laboratories | IMSR_JAX:000664 |
| Oligonucleotides | ||
| C-fos primer | Applied Biosystems | Mm00487425_m1 |
| Arc primer | Applied Biosystems | Mm01204954_g1 |
| GAPDH primer | Applied Biosystems | Mm99999915_g1 |
| Tubulin primer | Applied Biosystems | Mm00495806_g1 |
| Recombinant DNA | ||
| Wildtype ULK1 | Kun-Liang Guan | Kim et al., 2011 |
| ULK1 S757A | Kun-Liang Guan | Kim et al., 2011 |
| Software and Algorithms | ||
| Image J FIJI | NIH | https://imagej.net/; RRID:SCR_002285 |
| Graph Pad Prism | Graph Pad | https://www.graphpad.com/scientific-software/prism/;RRID:SCR_002798 |
| BZ Analyzer Software | Keyence | RRID:SCR_017205 |
| NeuroStudio | Icahn School of Medicine at Mount Sinai | RRID:SCR_013798 |
| Mini Analysis | Synaptosoft | RRID:SCR_002184 |
| Other | ||