Table 3.
Time scales and diffusion coefficients of Notch-relevant events.
| Timescale, diffusion coefficient | details | Time | Reference | Comment |
|---|---|---|---|---|
| Bond lifetime Notch1-Jag1, 0 pN | In vitro Notch1(8–12)-Jag(N-3) | 0.2s | (84) | Using BFP force clamp spectroscopy |
| Bond lifetime Notch1-Jag1, 9 pN | In vitro Notch1(8–12)-Jag(N-3) | ~0.5 s | (84) | Using BFP force clamp spectroscopy |
| Bond lifetime Notch1-Jag1V, 0 pN | In vitro Notch1(8–12)-Jag_JV1(N-3) | ~0.9 s | (84) | High affinity Jag1 variant using BFP force clamp spectroscopy |
| Bond lifetime Notch1-Dll4, 0 pN | In vitro Notch1(8–12)-Dll4(N-3) | ~0.15 s | (84) | Using BFP force clamp spectroscopy |
| Bond lifetime Notch1-Dll4, 9 pN | In vitro Notch1(8–12)-Dll4(N-3) | ~0.4 s | (84) | Using BFP force clamp spectroscopy |
| Clathrin mediated endocytosis | Mammalian cells | ~30 s | Reviewed in (70) | |
| Processing of Notch receptors | Mammalian cells | 15–30 sec | (119) | After applying 9 pN using nanoparticle |
| Appearance of NICD in the nucleus | Drosophila | ~10 min | (29) | Live imaging of Notch-GFP after asymmetric cell division |
| Appearance of NICD-RBPJ complexes in the nucleus | Mammalian cells | 5–10 min | (52) | Split luciferase system. |
| NICD half-life | Mammalian cells | 90–180 min | (52, 67) | Split luciferase system |
| Residence time of Su(H) on E(Sp) site in Notch-OFF | Drosophila - larval salivary glands | ~0.1–4 sec | (41) | FRAP on E(spl) locus |
| Residence time of Su(H) on E(Sp) site in Notch-ON | Drosophila - larval salivary glands | ~8–15 sec | (41) | FRAP on E(spl) locus |
| Transcriptional burst durations | c-elegans | 5–40 min | (76) | Live imaging using MS2-GFP. ON times vary with signal strength |
| Transcriptional burst duration | Drosophila embryo | 1–10 min | (35) | Live imaging using MS2-GFP. ON times vary with signal strength |
| Diffusion rate of Dll1 on cell membrane | Mammalian cells | 0.01 — 0.1 μm2/s | (58) | FRAP-TIRF imaging |
| Diffusion of Su(H) in the nucleus | Drosophila salivary gland | 0.1 − 2 μm2/s | (41) | Single particle tracking |